Benedict's test for sugars. Benedict's reagent is a chemical reagent that is used as a test for the presence of reducing sugars and aldehydes,and alpha-hydroxy-ketones (monosaccharides,lactose,maltose,fructose,etc). The reagent consists of sodium carbonate,sodium citrate and copper sulfate. Here the reagent (in the bottle) was added to two test tubes containing 20 g/L solutions of sucrose (table sugar) and glucose. After the tubes were placed in a hot water bath for few minutes,the colour of the non-reducing sugar (sucrose) solution did not change,while the colour of the reducing sugar (glucose) solution changed to brick-red/brown due to formation of copper oxide precipitate | |
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