Immunofluorescence bacteria detection. Animation showing two methods used to detect bacteria using immunofluoresence markers. The first part of the animation shows a bacterium (brown) with antigens (grey) on its surface. These antigens are the markers that enable larger molecules such as antibodies to bind to the bacterium. In this case, antibodies (green) that have been marked with a fluorescent dye (red) are shown binding to the antigens. The fluorescence allows the distribution of the labelled bacteria to be observed with a microscope. This direct labelling is known as the direct fluorescent antibody (DFA) test. A more indirect test involves the use of secondary antibodies (anti-antibodies) to bind to the original antibodies. This is shown in the second part of the animation, with the non-fluorescent antibodies (green) being bound to by anti-antibodies (pink) marked with a fluorescent dye (red). This secondary antibody testing is the method used in a wide range of analytical techniques such as the Western blot test and the ELISA test. For this animation with labels, see K004/4309. | |
Licence : | Droits gérés |
Crédit : | Science Photo Library / Biocosmos / Francis Leroy & Michael Pierard |
Model Release : | Non requis |
Durée : | 25 Secondes |
Aspect ratio : | 16/9 |
Restrictions : | - |